Expression of gastric-associated antigens by human premalignant and malignant colonic epithelial cells.

Abstract

A rabbit antiserum prepared to 2nd-trimester fetal organ extracts and absorbed with adult tissue (anti-STFa) was used to detect antigens common to 2nd-trimester fetal large bowel, normal adult stomach, several colon carcinoma cells (in primary and established cultures) and epithelial cells cultured from benign colonic polyps. The frequency of anti-STFa-positive cells was highest in cultures derived from benign tumors known to be associated with a greater degree of premalignancy. Thus, the percentage of antigen-positive cells increased from 18 and 43% to 70% of cultures from tubular, villotubular and villous adenomas, respectively. Considerable heterogeneity in the distribution of antigen-containing cells was evident within any given area of a positive culture. Absorption experiments, using a spectrum of fetal and normal adult tissue extracts, indicated that the adenoma-carcinoma specificity resides in fetal, but not adult, large bowel and normal adult stomach.

• Authors: P J Higgins, E Friedman, M Lipkin, R Hertz, F Attiyeh, E H Stonehill
• Journal: Oncology (Oncology) Vol. 40 Issue 1 Pg. 26-30 ( 1983) ISSN: 0030-2414 [Print] Switzerland
• PMID: 6185898 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Neoplasm
• Epitopes

Topics

• Adenoma (immunology)
• Antigens, Neoplasm (analysis, immunology)
• Cell Line
• Cells, Cultured
• Colonic Neoplasms (immunology)
• Epithelium (immunology)
• Epitopes (analysis)
• Female
• Fetus (immunology)
• Humans
• Polyps (immunology)
• Precancerous Conditions (immunology)
• Pregnancy
• Pregnancy Trimester, Second
• Stomach (immunology)