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Postmortem stability of dopamine-sensitive adenylate cyclase, guanylate cyclase, ATPase, and GTPase in rat striatum.

Abstract
The stability of dopamine-sensitive adenylate cyclase, guanylate cyclase, ATPase, and GTPase was measured in homogenates of rat striatal tissue frozen from 0 to 24 h postmortem. ATPase, GTPase, and Mg2+-dependent guanylate cyclase activities showed no significant change over this period. Mn2+-dependent guanylate cyclase activity was stable for 10 h postmortem. Basal and dopamine-stimulated adenylate cyclase activity decreased markedly during the first 5 h. However, when measured in washed membrane preparations, these adenylate cyclase activities remained stable for at least 10 h. Therefore, the postmortem loss of a soluble activator, such as GTP, may decrease the adenylate cyclase activity in homogenates. These results are not consistent with an earlier suggestion that there is a postmortem degradation of the enzyme itself. Other kinetic parameters of dopamine-sensitive adenylate cyclase can also be measured independently of postmortem changes. Thus, it is possible to investigate kinetic parameters of dopamine-sensitive adenylate cyclase, guanylate cyclase, ATPase, and GTPase in human brain obtained postmortem.
AuthorsS E Nicol, S E Senogles, T P Caruso, J J Hudziak, J D McSwigan, W H Frey 2nd
JournalJournal of neurochemistry (J Neurochem) Vol. 37 Issue 6 Pg. 1535-9 (Dec 1981) ISSN: 0022-3042 [Print] England
PMID6120996 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Adenylyl Imidodiphosphate
  • Guanylyl Imidodiphosphate
  • Phosphoric Monoester Hydrolases
  • Adenosine Triphosphatases
  • GTP Phosphohydrolases
  • Adenylyl Cyclases
  • Guanylate Cyclase
  • Magnesium
  • Dopamine
Topics
  • Adenosine Triphosphatases (metabolism)
  • Adenylyl Cyclases (metabolism)
  • Adenylyl Imidodiphosphate (pharmacology)
  • Animals
  • Corpus Striatum (enzymology, pathology)
  • Dopamine (pharmacology)
  • GTP Phosphohydrolases (metabolism)
  • Guanylate Cyclase (metabolism)
  • Guanylyl Imidodiphosphate (pharmacology)
  • Kinetics
  • Magnesium (pharmacology)
  • Male
  • Phosphoric Monoester Hydrolases (metabolism)
  • Postmortem Changes
  • Rats
  • Rats, Inbred Strains
  • Time Factors

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