Abstract |
The stability of dopamine-sensitive adenylate cyclase, guanylate cyclase, ATPase, and GTPase was measured in homogenates of rat striatal tissue frozen from 0 to 24 h postmortem. ATPase, GTPase, and Mg2+-dependent guanylate cyclase activities showed no significant change over this period. Mn2+-dependent guanylate cyclase activity was stable for 10 h postmortem. Basal and dopamine-stimulated adenylate cyclase activity decreased markedly during the first 5 h. However, when measured in washed membrane preparations, these adenylate cyclase activities remained stable for at least 10 h. Therefore, the postmortem loss of a soluble activator, such as GTP, may decrease the adenylate cyclase activity in homogenates. These results are not consistent with an earlier suggestion that there is a postmortem degradation of the enzyme itself. Other kinetic parameters of dopamine-sensitive adenylate cyclase can also be measured independently of postmortem changes. Thus, it is possible to investigate kinetic parameters of dopamine-sensitive adenylate cyclase, guanylate cyclase, ATPase, and GTPase in human brain obtained postmortem.
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Authors | S E Nicol, S E Senogles, T P Caruso, J J Hudziak, J D McSwigan, W H Frey 2nd |
Journal | Journal of neurochemistry
(J Neurochem)
Vol. 37
Issue 6
Pg. 1535-9
(Dec 1981)
ISSN: 0022-3042 [Print] England |
PMID | 6120996
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Adenylyl Imidodiphosphate
- Guanylyl Imidodiphosphate
- Phosphoric Monoester Hydrolases
- Adenosine Triphosphatases
- GTP Phosphohydrolases
- Adenylyl Cyclases
- Guanylate Cyclase
- Magnesium
- Dopamine
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Topics |
- Adenosine Triphosphatases
(metabolism)
- Adenylyl Cyclases
(metabolism)
- Adenylyl Imidodiphosphate
(pharmacology)
- Animals
- Corpus Striatum
(enzymology, pathology)
- Dopamine
(pharmacology)
- GTP Phosphohydrolases
(metabolism)
- Guanylate Cyclase
(metabolism)
- Guanylyl Imidodiphosphate
(pharmacology)
- Kinetics
- Magnesium
(pharmacology)
- Male
- Phosphoric Monoester Hydrolases
(metabolism)
- Postmortem Changes
- Rats
- Rats, Inbred Strains
- Time Factors
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