A study of the sulphur amino acids of rat tissues.

Abstract

1. In a study of the metabolism of l-[(35)S]methionine in vivo, the labelled sulphur compounds of rat liver and brain were separated first by ion-exchange chromatography into two fractions containing (i) free sulphur amino acids such as methionine, cystathionine, cyst(e)ine and homocyst(e)ine and (ii) glutathione. 2. Two-dimensional paper chromatography with butan-1-ol-acetic acid or propionic acid-water in the first direction and 80% acetone or acetone-ethyl methyl ketone-water in the second direction was found superior to other solvent systems for separating the sulphur amino acids. 3. At 10min. after injection of [(35)S]methionine only a small part of the (35)S was found combined in free methionine or other free sulphur amino acids. 4. Evidence was obtained of the presence of adenosyl[(35)S]methionine and adenosyl[(35)S]homocysteine in perchloric acid extracts of rat liver and brain. 5. The trans-sulphuration pathway was active in brain as well as in liver.

• Authors: G E Gaull, M K Gaitonde
• Journal: The Biochemical journal (Biochem J) Vol. 102 Issue 1 Pg. 294-303 (Jan 1967) ISSN: 0264-6021 [Print] England
• PMID: 6030290 (Publication Type: Journal Article)

Chemical References

• Amino Acids
• Sulfur Isotopes
• Homocysteine
• Methionine
• Glutathione
• Cysteine

Topics

• Amino Acids (analysis)
• Animals
• Animals, Newborn
• Brain (metabolism)
• Brain Chemistry
• Chromatography, Ion Exchange
• Chromatography, Paper
• Cysteine (analysis)
• Glutathione (analysis)
• Homocysteine (analysis)
• In Vitro Techniques
• Liver (analysis, metabolism)
• Male
• Methionine (analysis, metabolism)
• Rats
• Sulfur Isotopes