Quantitative thin-layer chromatographic measurement of n-trifluoroacetyladriamycin-14-valerate (AD 32) and trifluoroacetyladriamycin (AD 41) in blood and tissues.

Abstract

A thin-layer chromatographic method has been developed for the detection and measurement of N-trifluoroacetyladriamycin-14-valerate (AD 32) and its major metabolite trifluoroacetyladriamycin (AD 41). The procedure gives satisfactory linearity over a large range of concentrations. The coefficient of variability is about 10% over the entire range of usable concentrations, giving good reproducibility; sensitivity is 25 ng for both AD 32 and AD 41. Analysis is specific for AD 32 and AD 41 since adriamycin or more polar metabolites can be differentiated. Recovery is high (85-90%) and the method is simple and economical to use. Pharmacokinetics of AD 32 and AD 41 are reported in blood and some tissues of mice bearing Lewis Lung carcinoma.

• Authors: B Barbieri, R Abbruzzi, A Benigni, M Rizzardini, M G Donelli, S Garattini, M Salmona
• Journal: Journal of chromatography (J Chromatogr) Vol. 163 Issue 2 Pg. 195-200 (Jun 11 1979) Netherlands
• PMID: 541371 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Doxorubicin

Topics

• Animals
• Chromatography, Thin Layer (methods)
• Doxorubicin (analogs & derivatives, blood, pharmacology)
• Lung Neoplasms (blood)
• Male
• Mice
• Neoplasms, Experimental (blood)
• Tissue Distribution