Hemagglutination inhibition with arboviruses: relationship between titers and source of erythrocytes.

Abstract

Antigens for Grand Arbaud, Hazara, and California arboviruses were able to agglutinate goose and either dog, hamster and guinea pig, or hamster red blood cells (RBC) to the same titer at the same pH; in hemagglutination-inhibition (HI) tests, titers for homologous and related sera were the same with these different types of RBC or occasionally one dilution higher with the mammalian cells. Antigens for St. Louis encephalitis and Eastern equine encephalitis viruses required use of lower antigen dilutions with human, guinea pig, and hamster RBC than with goose RBC. The results of comparative HI testing with these latter antigens and types of RBC indicate that HI titer is not directly related to the antigen dilution used with different types of RBC.

• Authors: N E Mettler, D H Clarke, J Casals
• Journal: Applied microbiology (Appl Microbiol) Vol. 22 Issue 3 Pg. 377-9 (Sep 1971) ISSN: 0003-6919 [Print] United States
• PMID: 5165837 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antigens
• Antigens, Viral
• Immune Sera

Topics

• Animals
• Antigens (analysis)
• Antigens, Viral (analysis)
• Arboviruses (immunology)
• Ascitic Fluid
• Cricetinae
• Dogs
• Encephalitis Virus, St. Louis (immunology)
• Encephalitis Viruses (immunology)
• Erythrocytes (immunology)
• Geese
• Guinea Pigs
• Hemagglutination Inhibition Tests (standards)
• Hemagglutination Tests (standards)
• Humans
• Hydrogen-Ion Concentration
• Immune Sera
• Mice
• Rabbits
• Species Specificity