Superinfection with R factors by transduction in Escherichia coli and Salmonella typhimurium.

Abstract

Superinfection immunity is found in the conjugal transfer of R factors between two fi(+) R factors and between two fi(-) R factors (fi = fertility inhibition), as we reported previously. In contrast, no reduction in the frequencies of transduction of an fi(+) R factor 222 was caused by the presence of fi(+) R factors in the recipients in transduction systems with phage P1kc in Escherichia coli K-12 and with phage P22 in Salmonella typhimurium LT-2. The absence of superinfection immunity in transduction may be due to the difference in the route of entry of the R factor. The frequencies of transduction of an fi(+) R factor were reduced, although slightly, by the presence of fi(-) R factors in the recipients. This reduction is probably due to host-controlled restriction of the entering fi(+) R factor by the fi(-) R factors in the recipients, since transduction of an fi(+) R factor by the transducing phage propagated on the strain carrying both fi(+) and fi(-) R factors was not reduced by the presence of homologous fi(-) R factors in the recipients. The fi(+) R factor 222, when transduced to the recipient strains carrying other R factors, recombined genetically at high frequencies with these resident R factors, regardless of their fi type.

• Authors: T Watanabe, S Sakaizumi, C Furuse
• Journal: Journal of bacteriology (J Bacteriol) Vol. 96 Issue 5 Pg. 1796-802 (Nov 1968) ISSN: 0021-9193 [Print] United States
• PMID: 4882026 (Publication Type: Journal Article)

Topics

• Conjugation, Genetic
• Drug Resistance, Microbial
• Escherichia coli
• Extrachromosomal Inheritance
• Genetics, Microbial
• Recombination, Genetic
• Salmonella typhimurium
• Transduction, Genetic