Saturation behavior of ascites tumor cell chloride exchange in the presence of gluconate.

Abstract

Steady state Cl- flux across the Ehrlich mouse ascites cell membrane was studied when gluconate replaced Cl- in the external medium. Saturation behavior was observed; K 1/2 was 23.9 mM Cl- and V was 758 micromol.g-1 dry weight.h-1. The cells lost K+, Cl- and H2O, consistent with relative impermeability to gluconate, and the Cl- efflux rate coefficient was elevated. The results indicate that a major portion of Cl- exchange occurs as a membrane transport process and suggest that the process is sensitive to intracellular Cl- levels.

• Authors: F Aull
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 554 Issue 2 Pg. 538-40 (Jul 05 1979) ISSN: 0006-3002 [Print] Netherlands
• PMID: 486458 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Chlorides
• Gluconates
• Potassium

Topics

• Animals
• Biological Transport (drug effects)
• Carcinoma, Ehrlich Tumor (metabolism)
• Cell Membrane (drug effects, metabolism)
• Chlorides (metabolism)
• Gluconates (pharmacology)
• Kinetics
• Mice
• Potassium (metabolism)