Abstract |
As reported earlier, an intraperitoneal injection of 1 mug of endotoxin (ET) from Serratia marcescens rendered mice resistant against the nonspecific mouse ascites tumor TA3-Ha upon challenge 24 h after pretreatment with ET. Further studies were aimed at the elaboration of conditions which achieved maximal resistance. It appears that (i) a 10-mug dose of ET was approximately the optimal dose for protection; (ii) pretreatment with ET 3 to 0 days prior to tumor challenge gave best protection; and (iii) the intravenous injection of ET showed a lower protection against the tumor than intraperitoneal application. Studies on the mechanism of ET protection indicate that (i) ET does not have a direct cytotoxic effect on tumor cells; (ii) normal spleen cells exposed to ET in vitro can adoptively transfer protection against tumor; and (iii) spleen cells activated in vivo by intravenous injection of ET can adoptively transfer protection. The possible involvement of mononuclear cells is discussed.
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Authors | C Yang, A Nowotny |
Journal | Infection and immunity
(Infect Immun)
Vol. 9
Issue 1
Pg. 95-100
(Jan 1974)
ISSN: 0019-9567 [Print] United States |
PMID | 4587387
(Publication Type: Journal Article)
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Chemical References |
- Endotoxins
- Tissue Extracts
- Tritium
- Thymidine
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Topics |
- Animals
- Carcinoma, Ehrlich Tumor
(immunology)
- Cell Survival
- Dose-Response Relationship, Drug
- Endotoxins
(administration & dosage, pharmacology)
- Female
- Immunization, Passive
- In Vitro Techniques
- Injections, Intraperitoneal
- Injections, Intravenous
- Lymphocyte Activation
(drug effects)
- Lymphocytes
(drug effects)
- Mice
- Mice, Inbred C57BL
- Mollusca
- Neoplasm Transplantation
- Serratia marcescens
(immunology)
- Spleen
(cytology)
- Thymidine
(metabolism)
- Time Factors
- Tissue Extracts
- Tritium
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