HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Terminal deoxyribonucleotidyl transferase in human leukemia.

Abstract
Terminal deoxyribonucleotidyl transferase (EC 2.7.7.31; nucleoside triphosphate:DNA nucleotidylexotransferase) is usually found only in thymus, but has been reported in leukemic cells from children with acute lymphoblastic leukemia. In an unusual adult patient with acute myelomonocytic leukemia, terminal transferase was found at a level of 16 units per 10(8) bone marrow cells and 14 units per 10(8) circulating leukocytes (1 unit = 1 nmol of nucleotide per hr). This activity is comparable to that found in normal thymus. Assays of transferase in marrow and peripheral leukocytes from patients with typical acute and chronic myelogenous leukemias gave average values of 0.5 and 0.3 unit per 10(8) cells, respectively. Transferase activity is also found in normal bone marrow at about 0.07 unit per 10(8) cells. Terminal deoxyribonucleotidyl transferase in all samples of human marrow and peripheral blood had reaction characteristics, sedimentation, and chromatographic properties similar to the homogeneous enzyme from calf thymus.
AuthorsM S Coleman, J J Hutton, P De Simone, F J Bollum
JournalProceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 71 Issue 11 Pg. 4404-8 (Nov 1974) ISSN: 0027-8424 [Print] United States
PMID4530991 (Publication Type: Journal Article)
Chemical References
  • Tritium
  • Guanosine Triphosphate
  • DNA Nucleotidyltransferases
Topics
  • Aged
  • Bone Marrow (enzymology, pathology)
  • Bone Marrow Cells
  • DNA Nucleotidyltransferases (metabolism)
  • Female
  • Guanosine Triphosphate (metabolism)
  • Humans
  • Isoelectric Focusing
  • Leukemia, Myeloid (enzymology)
  • Leukemia, Myeloid, Acute (enzymology, pathology)
  • Leukocytes (enzymology)
  • Tritium

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: