A new extracorporeal reactor-dialyzer for enzyme therapy using immobilized L-asparaginase.

Abstract

Depletion of circulating L-asparagine has proved beneficial in the treatment of some acute lymphoyctic leukemias. To avoid the immunologic sequelae of administering L-asparaginase parenterally, we have covalently attached the enzyme to the outside of the fibers of a conventional hollow fiber hemodialyzer. This provides ready access of the substrate to the enzyme, while simultaneously isolating the foreign protein from the immune system. Such reactor-dialyzers perform well, both in vitro and in vivo. Circulating L-asparagine in the healthy dog is reduced from about 50 micrometer to less than 2 micrometer within 30 min of connecting the reactor-dialyzer and the reduction persists for at least 4 hr after cessation of treatment.

• Authors: J A Jackson, H R Halvorson, J W Furlong, K D Lucast, J D Shore
• Journal: The Journal of pharmacology and experimental therapeutics (J Pharmacol Exp Ther) Vol. 209 Issue 2 Pg. 271-4 (May 1979) ISSN: 0022-3565 [Print] United States
• PMID: 439002 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Enzymes, Immobilized
• Asparagine
• Cellulose
• Asparaginase

Topics

• Animals
• Asparaginase (therapeutic use)
• Asparagine (blood)
• Cellulose
• Dogs
• Enzymes, Immobilized (therapeutic use)
• Kidneys, Artificial
• Perfusion