Lithium-induced nephrogenic diabetes insipidus: in vivo and in vitro studies.

Abstract

The physiological basis for the polyuria and polydipsia occurring in some manic-depressive patients treated with lithium salts was studied in vivo and in vitro. Three lithium-treated polyuric patients, in whom other causes of a concentrating defect were excluded, had abnormal urinary concentrating abilities after a standard water depreviation test. Two of these patients failed to respond to exogenous vasopressin (ADH) and one had a subnormal response. The abilities of these patients to excrete solute-free water (C(H2O)) was comparable to normal subjects during steady-state water diuresis, suggesting no gross abnormalities in sodium transport. However, each of these patients demonstrated abnormally low capacities to reabsorb solute-free water (T(C) (H2O)) under hydropenic conditions after administration of hypertonic saline and vasopressin. These in vivo findings demonstrate at least a nephrogenic basis for the diabetes insipidus syndrome manifested by these three patients. The defect in water transport was further characterized in toad urinary bladders in vitro. Short-circuit current (I) and water flow (W) were studied under basal, ADH-stimulated, and cyclic adenosine 3',5'-monophosphate (c-AMP)-stimulated conditions. Increasing mucosal [Li(+)] progressively inhibited basal I, and both I and W induced by ADH. Significant inhibition of basal and ADH-induced I was observed at mucosal [Li(+)] < 1.1 mEq/liter, and of ADH-induced W at mucosal [Li(+)] = 11 mEq/liter. On the other hand, at these lithium concentrations, neither c-AMP-stimulated W nor I was inhibited. Increasing serosal [Li(+)] produced significant inhibition of basal I only at [Li(+)] at least 50-fold greater than at the mucosal (urinary) surface. These in vitro studies confirm that mucosal lithium inhibits the action of ADH, but not c-AMP. Hence, lithium appears to be a significant inhibitor of ADH-stimulated water flow, probably acts from the urinary surface, and appears to exert its effect at a site biochemically proximal to c-AMP action.

• Authors: I Singer, D Rotenberg, J B Puschett
• Journal: The Journal of clinical investigation (J Clin Invest) Vol. 51 Issue 5 Pg. 1081-91 (May 1972) ISSN: 0021-9738 [Print] United States
• PMID: 4341501 (Publication Type: Journal Article)

Chemical References

• Vasopressins
• Lithium
• Sodium
• Cyclic AMP

Topics

• Adult
• Animals
• Anura
• Biological Transport, Active
• Bipolar Disorder (drug therapy)
• Cyclic AMP
• Diabetes Insipidus (chemically induced)
• Drinking Behavior (drug effects)
• Humans
• In Vitro Techniques
• Kidney (drug effects)
• Kidney Concentrating Ability
• Lithium (adverse effects)
• Male
• Membrane Potentials
• Middle Aged
• Osmolar Concentration
• Polyuria (chemically induced)
• Sodium (metabolism)
• Urinary Bladder (drug effects)
• Vasopressins
• Water-Electrolyte Balance