Abstract |
phi 3T and rho11 are closely related bacteriophages of Bacillus subtilis which can "convent" thymine auxotrophs to thymine prototrophs upon infection or transfection. The effect of endonuclease digestion on the ability of both bacteriophage and prophage DNA from phi eT and rho11 to transform for thymine prototrophy was determined. All of the endonucleases tested: BamHI, Bg/II, BsuRI, EcoRI, HindII+ III, and HpaII reduced the efficiency of thyP transformation to an equal extent in prophage and bacteriophage DNA. Only HpaII completely abolished thyP transformation. The reduction in transformation with BamHI, Bg/II, BsuRI, EcoRI, and HpaII fragments is size related. The thyP transforming fragments generated by these endonucleases are potentially clonable.
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Authors | R S Graham, F E Young, G A Wilson |
Journal | Gene
(Gene)
Vol. 1
Issue 2
Pg. 169-80
(Mar 1977)
ISSN: 0378-1119 [Print] Netherlands |
PMID | 412726
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Bacillus subtilis
(genetics)
- Bacteriophages
(genetics)
- Endonucleases
(metabolism)
- Genes, Viral
- Thymine
(metabolism)
- Transformation, Genetic
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