The borohydride-reducible compounds of human aortic elastin. Demonstration of a new cyclic amino acid in alkali hydrolysate, and changes with age and in patients with annulo-aortic ectasia including one with Marfan syndrome.

Abstract

Human aortic elastin reduced with [3H]borohydride was analysed by ion-exchange chromatography after alkali or acid hydrolysis. Alkali hydrolysates of elastins contained a radioactive peak that was eluted between proline and leucine. This peak was not present in foetal elastin, but its proportion increased steadily during aging. Aortic samples from patients with annulo-aortic ectasia (aneurysm of the ascending aorta), including one with classical Marfan syndrome, contained less elastin (CNBr-insoluble material) than did the age-matched controls. The proportion of radioactivity in the new peak of all these aortas was low when compared with age-matched controls. Gas-chromatographic/mass-spectrometric analysis suggested that it contained a cyclic derivative of a hydrated aldol-condensation product. The concentration of the cross-link precursors, lysine aldehyde and aldol-condensation product (estimated from the acid-hydrolysis product 6-chloronorleucine and the acid-degradation product of reduced aldol-condensation product) was high in very young aortas but remained quite stable after childhood. No differences were observed in cross-link profiles of acid hydrolysates between pathological and control aortas. A low proportion of radioactivity in the new peak may indicate the presence of young or immature elastin in the pathological aortas.

• Authors: T Halme, M Jutila, T Vihersaari, P Oksman, N D Light, R Penttinen
• Journal: The Biochemical journal (Biochem J) Vol. 232 Issue 1 Pg. 169-75 (Nov 15 1985) ISSN: 0264-6021 [Print] England
• PMID: 4084226 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids
• Borohydrides
• sodium borohydride
• Elastin

Topics

• Adult
• Age Factors
• Amino Acids (analysis)
• Aorta (analysis)
• Aortic Diseases (metabolism)
• Borohydrides
• Child
• Chromatography, Ion Exchange
• Dilatation, Pathologic (metabolism)
• Elastin
• Female
• Gas Chromatography-Mass Spectrometry
• Humans
• Hydrolysis
• Male
• Marfan Syndrome (metabolism)
• Middle Aged