Use of a diode array detector in investigation of neonatal organic aciduria.

Abstract

We investigated the use of a diode array detector in conjunction with isocratic cation-exchange liquid chromatography for detection of organic acids in urine. The spectra and retention times of abnormal peaks found on chromatography of urine from patients with methylmalonic aciduria, maple syrup urine disease, and lactic aciduria were recorded and compared with those obtained for group of pure organic acids. Our results show that diode array detection helps considerably in tentatively identifying peaks appearing on an abnormal chromatogram while awaiting confirmation by mass spectrometry.

• Authors: K R Allen, R Khan, D Watson
• Journal: Clinical chemistry (Clin Chem) Vol. 31 Issue 4 Pg. 561-3 (Apr 1985) ISSN: 0009-9147 [Print] England
• PMID: 3978788 (Publication Type: Journal Article)

Chemical References

• Carboxylic Acids
• Lactates
• Lactic Acid
• Methylmalonic Acid

Topics

• Amino Acid Metabolism, Inborn Errors (urine)
• Carboxylic Acids (urine)
• Chromatography, Ion Exchange
• Electrochemistry
• Humans
• Infant, Newborn
• Lactates (urine)
• Lactic Acid
• Maple Syrup Urine Disease (urine)
• Metabolism, Inborn Errors (urine)
• Methylmalonic Acid (urine)