Abstract |
One of the proposed mechanisms of vascular damage in connective tissue disease is the direct action of a cytotoxic serum factor inducing endothelial cell damage. The nature of this serum factor is unclear, but has been suggested to be a lipoprotein. Sera from patients with (1) systemic necrotising arteritis ( polyarteritis nodosa, Wegener's granulomatosis, and necrotising arteritis associated with rheumatoid synovitis), (2) systemic or joint restricted rheumatoid disease, and (3) large vessel/ giant cell arteritis have been examined for cytotoxicity to human cultured endothelial cells and azide-resistant ferroxidase-like activity (indicative of the oxidised lipoprotein content). Stored sera from patients with necrotising arteritis showed a significantly enhanced tendency to develop oxidised lipoprotein, which correlated closely with human endothelial cell cytotoxicity. Fresh sera also contained this factor, but to a lesser extent. It is argued that the cytotoxic factor detected in previous clinical studies is in part an in-vitro artefact, although its accelerated development in certain patient groups might suggest an excess of pro-oxidants that have developed in vivo.
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Authors | D R Blake, P Winyard, D G Scott, S Brailsford, A Blann, J Lunec |
Journal | Annals of the rheumatic diseases
(Ann Rheum Dis)
Vol. 44
Issue 3
Pg. 176-82
(Mar 1985)
ISSN: 0003-4967 [Print] England |
PMID | 3977420
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Arteritis
(blood, immunology)
- Arthritis, Rheumatoid
(blood, immunology)
- Azides
(pharmacology)
- Cell Survival
- Cells, Cultured
- Cytotoxicity, Immunologic
- Endothelium
(cytology, immunology)
- Humans
- Lipoproteins
(blood, immunology)
- Oxidation-Reduction
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