Semiquantitative immunohistochemical evaluation of H-antigen expression in human ureters of different ABO- and Lewis types.

Abstract

A study was undertaken to obtain baseline information for future studies of H-antigen expression in urothelial tumors. The influence of ABO- and Lewis genes on H-antigen expression in human ureters was investigated. Thirty two ureters of different ABO- and Lewis types were stained by the Tween 20-modified immunoperoxidase staining technique using Ulex europaeus agglutinin I (32 cases) and two monoclonal anti-H antibodies (10 cases). Ureters from A and AB individuals were also stained using monoclonal anti-A and Dolichos biflorus agglutinin (14 cases). The H-antigen expression in endothelium of all cases and in urothelium of Lea-b+ and Lea-b- individuals was correlated to ABO blood type. H antigen was never detected in urothelium of Lea+b- individuals. Endothelial H-antigen expression was not influenced by the Lewis types. In A and AB individuals, urothelial and endothelial H- and A-antigen and Dolichos biflorus reactivities were mainly insignificantly related, with correlation coefficients ranging numerically from 0.10 to 0.72. The study stresses the importance of relating blood group antigen H expression in urothelial tumors to the ABO-, Lewis, and secretors types of the individual.

• Authors: B R Juhl
• Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (J Histochem Cytochem) Vol. 33 Issue 9 Pg. 867-74 (Sep 1985) ISSN: 0022-1554 [Print] United States
• PMID: 3894503 (Publication Type: Journal Article)

Chemical References

• ABO Blood-Group System
• Lewis Blood Group Antigens
• Phytohemagglutinins

Topics

• ABO Blood-Group System (immunology)
• Histocytochemistry
• Humans
• Immunoenzyme Techniques
• Lewis Blood Group Antigens (immunology)
• Methods
• Phytohemagglutinins
• Ureter (immunology)