Effect of model trauma on the turnover of protein and hemoprotein components of hepatic microsomal membrane in immature rats.

Abstract

Administration of 14C-leucine and delta-[3,5-3H]-aminolevulinic acid to immature male rats leads to the incorporation of radioactivity into microsomal protein, including the hemoprotein cytochrome P-450. Non-hepatic regional ischemic trauma results in an increase in the half-life of total microsomal protein, but does not exert the same effect on microsomal heme-associated protein. Loss of radioactivity from microsomal hemoprotein, primarily cytochrome P-450, from traumatized animals exhibits a biphasic pattern similar to that in control animals. The half-life of both the fast-phase component and the slow-phase component is unchanged by trauma. Trauma does, however, increase the ratio of the fast- to slow-phase components of microsomal heme. A significant increase in heme oxygenase activity after trauma suggests that the fast-phase component of hepatic microsomal cytochrome P-450 is more extensively degraded.

• Authors: J D George, B M Sadler, G M Rosen, E J Rauckman
• Journal: Developmental pharmacology and therapeutics (Dev Pharmacol Ther) Vol. 8 Issue 4 Pg. 243-53 ( 1985) ISSN: 0379-8305 [Print] Switzerland
• PMID: 3839742 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Hemeproteins
• Lipid Peroxides
• Proteins
• Cytochrome P-450 Enzyme System
• Heme Oxygenase (Decyclizing)

Topics

• Animals
• Animals, Suckling
• Cytochrome P-450 Enzyme System (metabolism)
• Half-Life
• Heme Oxygenase (Decyclizing) (metabolism)
• Hemeproteins (metabolism)
• Intracellular Membranes (metabolism)
• Ischemia (metabolism)
• Lipid Peroxides (metabolism)
• Male
• Microsomes, Liver (metabolism)
• Proteins (metabolism)
• Rats
• Rats, Inbred Strains
• Wounds and Injuries (metabolism)