HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Isolation and characterization of genomic and cDNA clones of human erythropoietin.

Abstract
The glycoprotein hormone erythropoietin regulates the level of oxygen in the blood by modulating the number of circulating erythrocytes, and is produced in the kidney or liver of adult and the liver of fetal or neonatal mammals. Neither the precise cell types that produce erythropoietin nor the mechanisms by which the same or different cells measure the circulating oxygen concentration and consequently regulate erythropoietin production are known. Cells responsive to erythropoietin have been identified in the adult bone marrow, fetal liver or adult spleen. In cultures of erythropoietic progenitors, erythropoietin stimulates proliferation and differentiation to more mature red blood cells. Detailed molecular studies have been hampered, however, by the impurity and heterogeneity of target cell populations and the difficulty of obtaining significant quantities of the purified hormone. Highly purified erythropoietin may be useful in the treatment of various forms of anaemia, particularly in chronic renal failure. Here we describe the cloning of the human erythropoietin gene and the expression of an erythropoietin cDNA clone in a transient mammalian expression system to yield a secreted product with biological activity.
AuthorsK Jacobs, C Shoemaker, R Rudersdorf, S D Neill, R J Kaufman, A Mufson, J Seehra, S S Jones, R Hewick, E F Fritsch
JournalNature (Nature) 1985 Feb 28-Mar 6 Vol. 313 Issue 6005 Pg. 806-10 ISSN: 0028-0836 [Print] England
PMID3838366 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Erythropoietin
  • DNA
Topics
  • Amino Acid Sequence
  • Base Sequence
  • Biological Assay
  • Cells, Cultured
  • Cloning, Molecular
  • DNA (genetics)
  • Erythropoietin (genetics)
  • Gene Expression Regulation
  • Genes
  • Humans
  • Liver (embryology)
  • Protein Processing, Post-Translational

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: