Ultrastructural and enzyme histochemical studies in orotic acid-induced fatty liver.

Abstract

To confirm the relationship between fatty changes and zonal distribution in the hepatic lobules, we studied the ultrastructural and enzyme histochemical alterations in rat hepatocytes of orotic acid which blocks the release of lipoproteins, administered for 7 days, fatty changes were clearly detected in the hepatocytes, but no zonal distribution of fat in the hepatic lobules was detected. The most striking ultrastructural alterations were observed in the rough endoplasmic reticulum and Golgi complexes. The intensity of the staining of non-specific esterase and lipase was markedly decreased. Based on these data, functional aspects of the hepatocytes in orotic acid administration were discussed.

• Authors: M Murakoshi, M Misawa, K Watanabe
• Journal: The Tokai journal of experimental and clinical medicine (Tokai J Exp Clin Med) Vol. 11 Issue 1 Pg. 39-45 (Mar 1986) ISSN: 0385-0005 [Print] Japan
• PMID: 3810692 (Publication Type: Journal Article)

Chemical References

• Orotic Acid
• Esterases
• Lipase

Topics

• Animals
• Esterases (metabolism)
• Fatty Liver (chemically induced, enzymology, pathology)
• Histocytochemistry
• Lipase (metabolism)
• Liver (enzymology, ultrastructure)
• Male
• Microscopy, Electron
• Orotic Acid
• Rats
• Rats, Inbred Strains