A rapid slide agglutination test for the serodiagnosis of Brucella canis infection that employs a variant (M-) organism as antigen.

Abstract

An improved antigen is described for use in the serodiagnosis of canine brucellosis. The novel antigen employs a less mucoid (M-) variant of B. canis. The replacement of Brucella ovis with B. canis (M-) cells as antigen in slide agglutination tests would increase accuracy by reducing the rate of false positive results from ca. 50% to ca. 10%. Rose bengal stained B. canis (M-) cells are slightly more sensitive than the commercially available Brucella ovis as slide test antigens. Both test procedures require brief (less than 1 min) reaction with 2-mercaptoethanol in order to reduce the rate of false positive reactions.

• Authors: L E Carmichael, J C Joubert
• Journal: The Cornell veterinarian (Cornell Vet) Vol. 77 Issue 1 Pg. 3-12 (Jan 1987) ISSN: 0010-8901 [Print] United States
• PMID: 3802828 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial

Topics

• Agglutination Tests (veterinary)
• Animals
• Antigens, Bacterial (immunology)
• Brucella (immunology)
• Brucellosis (diagnosis, veterinary)
• Dog Diseases (diagnosis, microbiology)
• Dogs