Nucleotide sequence of the gene encoding the matrix protein of Newcastle disease virus.

Abstract

The nucleotide sequence of the gene encoding the matrix (M) protein of the Beaudette C strain of Newcastle disease virus (NDV) has been determined from overlapping cDNA clones. Control sequences typical of paramyxovirus mRNA start and polyadenylation signals have been identified. Assuming that the M gene starts and finishes at these sequences, the M gene is 1241 nucleotides long and encodes one long open reading frame of 364 amino acids, corresponding to a polypeptide of molecular weight 39605, in good agreement with estimates from SDS gels. The M protein has an amino acid sequence that is both hydrophobic and highly basic. The NDV M protein has sequence homologies to the M proteins of Sendai, measles, canine distemper and respiratory syncytial viruses.

• Authors: P Chambers, N S Millar, S G Platt, P T Emmerson
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 14 Issue 22 Pg. 9051-61 (Nov 25 1986) ISSN: 0305-1048 [Print] England
• PMID: 3786143 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Viral Matrix Proteins
• Viral Proteins
• DNA

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA (metabolism)
• Genes
• Genes, Viral
• Newcastle disease virus (genetics)
• Species Specificity
• Viral Matrix Proteins
• Viral Proteins (genetics)