Abstract |
The optical aberrations due to the presence of the turbidity caused by hyperlipidemia has been eliminated in two serum glucose procedures. This has been accomplished by incorporating lipase and alpha-cyclodextrin into the two glucose reagents. The hydrolytic action of the lipase generates water soluble glycerol and insoluble fatty acids. By including the chemical scavenger alpha-cyclodextrin into the reagent the fatty acids are solubilized and thus the production of a second turbidity is avoided. Because the clearing reagents are incorporated into the glucose reagents, this is an on-line process and no additional labor is required to clear the sample- reagent mixture. Furthermore, no additional time is required as the clearing occurs in the same period of time that it takes for the indicator reactions to reach equilibrium.
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Authors | J D Artiss, D R Strandbergh, A Sharma, B Zak |
Journal | Clinica chimica acta; international journal of clinical chemistry
(Clin Chim Acta)
Vol. 156
Issue 2
Pg. 151-6
(Apr 30 1986)
ISSN: 0009-8981 [Print] Netherlands |
PMID | 3708861
(Publication Type: Journal Article)
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Chemical References |
- Blood Glucose
- Cyclodextrins
- Fatty Acids, Nonesterified
- Indicators and Reagents
- Triglycerides
- alpha-Cyclodextrins
- Glucose Oxidase
- Hexokinase
- Lipase
- alpha-cyclodextrin
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Topics |
- Blood Glucose
(analysis)
- Cyclodextrins
- Fatty Acids, Nonesterified
(blood)
- Glucose Oxidase
- Hexokinase
- Humans
- Hyperlipidemias
(blood)
- Indicators and Reagents
- Lipase
- Spectrophotometry
- Triglycerides
(blood)
- alpha-Cyclodextrins
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