Diagnosis of storage pool deficiency by determination of diadenosine 5', 5'''-P1,P4-tetraphosphate in whole blood.

Platelets from cat and cattle with Chediak-Higashi disease were found completely devoid of Ap4A as measured by high performance liquid chromatography. Using a very sensitive firefly biolumnescence method 6% of the normal content of Ap4A was, however, found in platelets from sick animals. A content of Ap A of 1.90 +/- 0.11 X 10 M (means +/- SEM, n = 10) was found in whole normal human blood as measured by firefly bioluminescense method in trichloroacetic acid extracts of the blood samples. This concentration corresponds to the contribution from the platelets, thus the contribution of Ap4A from erythrocytes and the "buffy-coat" is negligible. Using the same method an Ap4A contents in platelets of 0.063 and 0.021 nmol/mg of protein compared to the normal content of 0.42 nmol/mg of protein (1) was found in two patients with severe myeloproliferative disorder calculated in this way on basis of platelet counts and on the assumption that 10(11) platelets contain 189 mg of protein (2). Comparison of these figures with parallel HPLC analyses on acid extracts of platelets isolated from the same patient were in agreement. The storage pool deficiency of adenine nucleotides in this disease found by others on basis of release experiments (3) can thus be diagnosed by rapid and simple measurements of Ap4A in whole blood using the advantage of Ap4A being a specific components stored in dense granules.
AuthorsH Flodgaard, P C Zamecnik, K Meyers, H Klenow
JournalThrombosis research (Thromb Res) Vol. 41 Issue 3 Pg. 345-51 (Feb 1 1986) ISSN: 0049-3848 [Print] UNITED STATES
PMID3705012 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adenine Nucleotides
  • Dinucleoside Phosphates
  • diadenosine tetraphosphate
  • Adenine Nucleotides (blood)
  • Animals
  • Blood Platelet Disorders (blood)
  • Blood Platelets (metabolism)
  • Cats
  • Cattle
  • Chediak-Higashi Syndrome (blood)
  • Cytoplasmic Granules (metabolism)
  • Dinucleoside Phosphates
  • Humans
  • Luminescent Measurements
  • Platelet Storage Pool Deficiency (blood, diagnosis)

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