Linear response curves from an ELISA assay: measurement of anti-myelin IgG and IgM during experimental allergic neuritis.

Abstract

An ELISA method for generating linear dose-response curves for measuring anti-myelin IgG and IgM is described. Linearity is achieved without logarithmic transformations. This test is used to measure plasma levels of anti-myelin antibodies in rabbits with EAN. Results are expressed as relative concentrations of antibody in arbitary mass units with 95% confidence limits fitted by inverse prediction. In the 8 animals studied, appearance of anti-myelin antibody always preceded onset of clinical signs and neither anti-myelin IgG nor IgM could be detected in any animal pre-inoculation. Five out of 8 animals displayed steady and progressive increases in anti-myelin IgG with the remaining 3 animals showing plateaus in IgG levels 21-30 days post-inoculation. Increases in anti-myelin IgG were generally parallelled by increases in disease severity. However, 2 animals showed recovery and relapse from EAN with no change in plasma levels of anti-myelin IgG. Patterns of production of anti-myelin IgM varied with some animals displaying early peaks while others showed gradual increases.

• Authors: G K Harvey, K Schindhelm, J H Antony, J D Pollard
• Journal: Journal of neuroscience methods (J Neurosci Methods) Vol. 21 Issue 1 Pg. 81-90 (Sep 1987) ISSN: 0165-0270 [Print] Netherlands
• PMID: 3657272 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Immunoglobulin G
• Immunoglobulin M

Topics

• Animals
• Antibodies (analysis)
• Enzyme-Linked Immunosorbent Assay
• Female
• Immunoglobulin G (analysis)
• Immunoglobulin M (analysis)
• Myelin Sheath (immunology)
• Neuritis, Autoimmune, Experimental (immunology)
• Rabbits