Anaerobic bacteria in tropical ulcer--the application of a new transport system for their isolation.

Abstract

The successful isolation of anaerobes largely depends on adequate transportation of the specimens after collection. We are investigating the role of anaerobes in the pathogenesis of tropical ulcer and have used Hungate tubes with pre-reduced peptone yeast broth and agar as a transportation system. The medium included 0.05% cysteine and 0.03% sodium formaldehyde sulphoxylate as an Eh buffer. Resazurin 1 microgram/ml seved as an Eh indicator. We found the tubes convenient to use and very reliable at maintaining anaerobiasis.

• Authors: B Adriaans, R J Hay, B S Drasar, D C Robinson
• Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene (Trans R Soc Trop Med Hyg) Vol. 80 Issue 5 Pg. 793-4 ( 1986) ISSN: 0035-9203 [Print] England
• PMID: 3603618 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media

Topics

• Adolescent
• Adult
• Anaerobiosis
• Bacteria, Anaerobic (isolation & purification)
• Child
• Child, Preschool
• Culture Media
• Humans
• Skin Ulcer (microbiology)
• Specimen Handling (methods)
• Zambia