Abstract |
A model system with human colonic grafts containing carcinoembryonic antigen (CEA) in nude mice was used to improve specificity and sensitivity of the radioimmunolocalization method. Monoclonal antibody MAb I-38S1 and monkey anti-CEA appeared to have high in vitro and in vivo specificity. Fab fragments of MAb I-38S1 improved localization ratios. A reduced time for optimal immunolocalization was obtained with Fab compared with MAb of the same specificity. Combinations of Fab:s, vasoactive compounds or anti-IgG treatment to reduce background levels did not further improve speed of localization or sensitivity.
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Authors | E Ljungdahl-Ståhle, U Harmenberg, U Rudén, S Hammarström, T Stigbrand, B Wahren |
Journal | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
(Tumour Biol)
Vol. 7
Issue 5-6
Pg. 353-60
( 1986)
ISSN: 1010-4283 [Print] Netherlands |
PMID | 3576081
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Carcinoembryonic Antigen
- GPI-Linked Proteins
- Immunoglobulin Fab Fragments
- Iodine Radioisotopes
- Isoenzymes
- Alkaline Phosphatase
- alkaline phosphatase, placental
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Topics |
- Alkaline Phosphatase
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Carcinoembryonic Antigen
(analysis, immunology)
- Carcinoma
(diagnosis, immunology)
- GPI-Linked Proteins
- Humans
- Immunoglobulin Fab Fragments
(immunology)
- Iodine Radioisotopes
- Isoenzymes
(immunology)
- Mice
- Mice, Inbred BALB C
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