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Improved radioimmunolocalization of carcinomas using Fab fragments with anti-CEA specificity.

Abstract
A model system with human colonic grafts containing carcinoembryonic antigen (CEA) in nude mice was used to improve specificity and sensitivity of the radioimmunolocalization method. Monoclonal antibody MAb I-38S1 and monkey anti-CEA appeared to have high in vitro and in vivo specificity. Fab fragments of MAb I-38S1 improved localization ratios. A reduced time for optimal immunolocalization was obtained with Fab compared with MAb of the same specificity. Combinations of Fab:s, vasoactive compounds or anti-IgG treatment to reduce background levels did not further improve speed of localization or sensitivity.
AuthorsE Ljungdahl-Ståhle, U Harmenberg, U Rudén, S Hammarström, T Stigbrand, B Wahren
JournalTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (Tumour Biol) Vol. 7 Issue 5-6 Pg. 353-60 ( 1986) ISSN: 1010-4283 [Print] Netherlands
PMID3576081 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Carcinoembryonic Antigen
  • GPI-Linked Proteins
  • Immunoglobulin Fab Fragments
  • Iodine Radioisotopes
  • Isoenzymes
  • Alkaline Phosphatase
  • alkaline phosphatase, placental
Topics
  • Alkaline Phosphatase
  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Carcinoembryonic Antigen (analysis, immunology)
  • Carcinoma (diagnosis, immunology)
  • GPI-Linked Proteins
  • Humans
  • Immunoglobulin Fab Fragments (immunology)
  • Iodine Radioisotopes
  • Isoenzymes (immunology)
  • Mice
  • Mice, Inbred BALB C

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