Abstract |
Monoclonal antibodies (McAbs) were prepared against carcinoembryonic antigen (CEA), derived from human metastases to the liver of primary colon adenocarcinoma. A stable clone, secreting monoclonal anti-CEA antibody (Ab), was selected. The hybridoma cells were grown in BALB/C mice, forming ascites fluids containing 2-5 mg/ml of the relevant McAbs. Ascites fluids were purified using a DEAE-Sephacel column. The purified immunoglobulin fraction was characterized as IgG2b, having a dissociation constant of 2 X 10(-9)M. The potential use of these McAbs for CEA diagnosis and purification was evaluated: the IgG2b fraction, when bound to Sepharose 4B, facilitated the purification of CEA from spent media of a human colon adenocarcinoma cell line, resulting in iodination grade CEA; the anti-CEA McAbs demonstrated differential histochemical staining of various sections, both fresh and fixed; the McAbs were also found to be suitable as tagging Abs in a CEA RIA.
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Authors | N Epstein, R Zandani, I Inbar, Z Altbaum, A Lazar, S Reuveny, J Geva, D Marcus, A Mizrahi, N Ariel |
Journal | Developments in biological standardization
(Dev Biol Stand)
Vol. 66
Pg. 429-37
( 1987)
ISSN: 0301-5149 [Print] Switzerland |
PMID | 3556272
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Carcinoembryonic Antigen
- Culture Media
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Topics |
- Adenocarcinoma
(metabolism)
- Antibodies, Monoclonal
- Carcinoembryonic Antigen
(isolation & purification)
- Cell Line
- Chromatography, Affinity
(methods)
- Colonic Neoplasms
(metabolism)
- Culture Media
(analysis)
- Humans
- Immunosorbent Techniques
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