Human allospecific cytolytic T lymphocyte lysis of a murine cell transfected with HLA-A2.

Abstract

A variety of molecules are involved in the interaction of human allospecific cytolytic T lymphocytes (CTL) with target cells. Monoclonal antibodies specific for these molecules inhibit CTL-target conjugate formation and/or lysis. To further study recognition and lysis of targets by human CTL, we used a murine mastocytoma cell line transfected with the histocompatibility leukocyte antigen (HLA)-A2 gene (P815-A2+) as a target for human HLA-A2-specific CTL. We find that only a subset of human HLA-A2-specific CTL can lyse murine P815-A2+ cells, suggesting that the murine cells may lack one or more accessory molecules needed for CTL recognition and lysis.

• Authors: T D Koller, C Clayberger, J L Maryanski, A M Krensky
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 138 Issue 7 Pg. 2044-9 (Apr 01 1987) ISSN: 0022-1767 [Print] United States
• PMID: 3549894 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, Surface
• HLA Antigens
• HLA-A2 Antigen
• Lymphocyte Function-Associated Antigen-1

Topics

• Animals
• Antibodies, Monoclonal
• Antigens, Surface (immunology)
• Cell Line
• Cytotoxicity, Immunologic
• Flow Cytometry
• HLA Antigens (analysis, genetics, immunology)
• HLA-A2 Antigen
• Humans
• Immunity, Cellular
• Lymphocyte Function-Associated Antigen-1
• Mice
• T-Lymphocytes, Cytotoxic (immunology)
• Transfection