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Differentiation-associated decrease in muscarinic receptor sensitivity in human neuroblastoma cells.

Abstract
Muscarinic receptor-linked increases in intracellular free Ca2+ as measured with quin-2 and Ca2+ release from monolayers of cells have been measured in the human neuroblastoma cell line SH-SY5Y. Induction of differentiation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to a decrease in the sensitivity of the cells to low concentrations of agonists with respect to the induced increase in cytosolic free Ca2+ and stimulation of Ca2+ efflux. No decrease in agonist binding affinity was observed when the displacement of a labelled antagonist, 3H-NMS, by a non-labelled agonist was studied.
AuthorsJ E Heikkilä, I G Scott, L A Suominen, K E Akerman
JournalJournal of cellular physiology (J Cell Physiol) Vol. 130 Issue 1 Pg. 157-62 (Jan 1987) ISSN: 0021-9541 [Print] United States
PMID3543028 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Methacholine Compounds
  • Receptors, Muscarinic
  • Scopolamine Derivatives
  • Methacholine Chloride
  • Calcium
  • N-Methylscopolamine
Topics
  • Calcium (metabolism)
  • Cell Differentiation
  • Cell Line
  • Humans
  • Kinetics
  • Methacholine Chloride
  • Methacholine Compounds (pharmacology)
  • N-Methylscopolamine
  • Neuroblastoma (metabolism, pathology)
  • Receptors, Muscarinic (metabolism)
  • Scopolamine Derivatives (metabolism)

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