Abstract |
Muscarinic receptor-linked increases in intracellular free Ca2+ as measured with quin-2 and Ca2+ release from monolayers of cells have been measured in the human neuroblastoma cell line SH-SY5Y. Induction of differentiation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to a decrease in the sensitivity of the cells to low concentrations of agonists with respect to the induced increase in cytosolic free Ca2+ and stimulation of Ca2+ efflux. No decrease in agonist binding affinity was observed when the displacement of a labelled antagonist, 3H-NMS, by a non-labelled agonist was studied.
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Authors | J E Heikkilä, I G Scott, L A Suominen, K E Akerman |
Journal | Journal of cellular physiology
(J Cell Physiol)
Vol. 130
Issue 1
Pg. 157-62
(Jan 1987)
ISSN: 0021-9541 [Print] United States |
PMID | 3543028
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Methacholine Compounds
- Receptors, Muscarinic
- Scopolamine Derivatives
- Methacholine Chloride
- Calcium
- N-Methylscopolamine
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Topics |
- Calcium
(metabolism)
- Cell Differentiation
- Cell Line
- Humans
- Kinetics
- Methacholine Chloride
- Methacholine Compounds
(pharmacology)
- N-Methylscopolamine
- Neuroblastoma
(metabolism, pathology)
- Receptors, Muscarinic
(metabolism)
- Scopolamine Derivatives
(metabolism)
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