Detection of IgM antibodies against rubella virus: comparison of two indirect ELISAs and an anti-IgM capture immunoassay.

Abstract

A commercial antibody capture enzyme immunoassay (Rubenz M) was compared to two commercial indirect enzyme immunoassays (Enzygnost IgM, Rubazyme-M) for the detection of rubella-specific IgM. Five hundred and fifty-two sera collected between the day of onset and 272 days after the onset of the exanthem of primary rubella were tested. Rubenz M was more sensitive early and late after the onset of the exanthem than the two indirect ELISAs. Rubenz M also appeared more sensitive when 240 sera were examined from patients with possible rubella in pregnancy, reinfection in pregnancy, suspected intrauterine infection, and recent vaccination. However, 5.5% of 968 pregnant women with no history of rubellalike symptoms or recent vaccination, the majority with elevated HAI titers, gave a low-positive or borderline result with Rubenz M. None of these women delivered a congenitally infected child. Therefore, borderline and low-positive results must be interpreted with caution, as for any assay for rubella-specific IgM.

• Authors: G Enders, F Knotek
• Journal: Journal of medical virology (J Med Virol) Vol. 19 Issue 4 Pg. 377-86 (Aug 1986) ISSN: 0146-6615 [Print] United States
• PMID: 3528389 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antibodies, Viral
• Immunoglobulin M
• Reagent Kits, Diagnostic
• Rheumatoid Factor

Topics

• Antibodies, Viral (analysis)
• Child
• Enzyme-Linked Immunosorbent Assay
• False Positive Reactions
• Female
• Humans
• Immunoenzyme Techniques
• Immunoglobulin M (analysis)
• Pregnancy
• Pregnancy Complications, Infectious (diagnosis)
• Reagent Kits, Diagnostic
• Rheumatoid Factor
• Rubella (diagnosis)
• Rubella virus (immunology)