Abstract |
A 2-yr-old girl with a nonsyphilitic type of paroxysmal cold hemoglobinuria (PCH) is reported. We applied the plaque-forming cell (PFC) assay to the measurement of Donath-Landsteiner ( DL) antibody-producing cells in the patient. Some of the circulating B lymphocytes formed plaques in our assay system. Initially the number of PFC was 1442 +/- 225/10(6) lymphocytes and DL titer was 1:16. Thereafter the PFC decreased in number as the DL titer decreased. Eight weeks later circulating PFC were not found but the DL titer was still 1:1 positive. The PFC assay appears to be useful as a new method for obtaining rapid imformation as to the disease status of PCH.
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Authors | Y Miyagawa, S Yamada, A Komiyama, T Akabane |
Journal | Blood
(Blood)
Vol. 52
Issue 1
Pg. 97-101
(Jul 1978)
ISSN: 0006-4971 [Print] United States |
PMID | 350317
(Publication Type: Case Reports, Journal Article)
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Topics |
- Antibody-Producing Cells
(immunology)
- B-Lymphocytes
(immunology)
- Child, Preschool
- Female
- Hemoglobinuria, Paroxysmal
(immunology)
- Hemolytic Plaque Technique
- Humans
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