Critical assessment of four monoclonal antibodies reactive with B-cells in formalin-fixed paraffin-embedded tissues.

Abstract

Four commercially available monoclonal antibodies, MB1, MB2, LN1 and LN2, were studied to determine their sensitivity and specificity for the diagnosis of B-cell lymphomas when used on formalin-fixed paraffin-embedded tissues. In addition to 125 cases of immunologically characterized non-Hodgkin's lymphoma, a range of normal tissues, reactive lymphoid proliferations, Hodgkin's disease and granulocytic sarcomas were also studied. MB1 was found to give positive results in 53.6% of B-cell lymphomas, but the staining was sometimes weak and patchy; there was also cross-reaction with 1.8% of T-cell lymphomas. MB2 reacted with 88.4% of B-cell lymphomas and the reaction was often strong and diffuse, but it showed cross-reaction with 18.2% of T-cell lymphomas. LN1 and LN2 gave positive staining of 44.9 and 46.4% of B-cell lymphomas respectively, and the results appeared to be inferior to that obtained in B5-fixed tissues; staining was sometimes weak and focal, and they also gave false-positive results in a few cases of T-cell lymphoma. This study shows that MB1, LN1 and LN2 are fairly but not entirely specific for B-cells in the non-Hodgkin's lymphomas, but are not very sensitive when applied to formalin-fixed tissues. MB2 shows a high sensitivity but only moderate specificity. Therefore, when these antibodies are used to determine the immunophenotype of malignant lymphomas, the B-cell nature can be predicted with great confidence only when two, preferably three or more, of the antibodies give positive results. The potential applications of these antibodies are discussed.

• Authors: C S Ng, J K Chan, S T Lo, D S Lo
• Journal: Histopathology (Histopathology) Vol. 11 Issue 12 Pg. 1243-58 (Dec 1987) ISSN: 0309-0167 [Print] England
• PMID: 3501985 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal

Topics

• Antibodies, Monoclonal
• B-Lymphocytes (immunology)
• Hodgkin Disease (immunology, pathology)
• Humans
• Leukemia, Myeloid (immunology, pathology)
• Lymphoid Tissue (immunology, pathology)
• Lymphoma (immunology, pathology)
• Lymphoma, Non-Hodgkin (immunology, pathology)
• Tissue Preservation