Abstract |
An attempt has been made to generate monoclonal antibodies which recognize the same target structures on influenza-infected cells as those seen by cytotoxic T lymphocyte (CTL) receptors. Such antibodies, if they mimicked the T cell receptor specificity, would be expected to be both virus specific and restricted in their binding by the major histocompatibility complex (MHC) antigens. Approximately 200 hybridomas from C57BL/6 (H-2b) mice primed and boosted with influenza virus (X-31)-infected EL4 (a C57BL/6 T cell lymphoma) were screened for reactivity on infected and uninfected cells of different MHC haplotypes. Of the 10 hybridoma antibodies which were identified as being reactive with X-31-infected EL4, but not uninfected EL4, all reacted equally well with X-31-infected cells of H-2b, H-2d and H-2k haplotypes, indicating a lack of MHC restriction in their recognition of the infected cells. Unexpectedly, 7 of the 10 monoclonal antibodies were found to react specifically with the purified influenza virus nucleoprotein (NP), a predominant viral antigen in CTL recognition of infected cells. Fluorescence-activated flow cytometry confirmed that these antibodies were able to recognize NP serological determinants on the surface of viable, infected cells, but the anti-NP antibodies were unable to block the lytic activity of an NP-specific CTL clone.
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Authors | W L Tamminen, D Wraith, B H Barber |
Journal | European journal of immunology
(Eur J Immunol)
Vol. 17
Issue 7
Pg. 999-1006
(Jul 1987)
ISSN: 0014-2980 [Print] Germany |
PMID | 3497045
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antibodies, Viral
- Antigens, Surface
- NP protein, Influenza A virus
- Nucleocapsid Proteins
- Nucleoproteins
- RNA-Binding Proteins
- Viral Core Proteins
- Viral Proteins
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Topics |
- Animals
- Antibodies, Monoclonal
(biosynthesis, immunology)
- Antibodies, Viral
(immunology)
- Antibody Formation
- Antibody Specificity
- Antigens, Surface
(analysis)
- Cytotoxicity, Immunologic
- Enzyme-Linked Immunosorbent Assay
- Flow Cytometry
- Haplotypes
- Hybridomas
(metabolism)
- Immunization, Passive
- Influenza A virus
(immunology)
- Major Histocompatibility Complex
- Mice
- Mice, Inbred Strains
(immunology)
- Nucleocapsid Proteins
- Nucleoproteins
(immunology)
- RNA-Binding Proteins
- Radioimmunoassay
- Spleen
(cytology, immunology)
- T-Lymphocytes, Cytotoxic
(immunology)
- Viral Core Proteins
- Viral Proteins
(immunology)
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