Detection of cytotoxicity of freshly obtained lymphocytes and of lymphocytes activated with recombinant interleukin II (rIL-2) against lung cancer cell lines by human tumor clonogenic assay (HTCA).

Abstract

The cytotoxicity of freshly obtained human peripheral blood lymphocytes and lymphocytes activated with human recombinant interleukin II (rIL-2) was evaluated against lung cancer cell lines by the human tumor clonogenic assay. Colony formation of all human lung cancer cell lines, PC-1, 3, 6, 7, 9, 13 and 14 were suppressed by lymphocytes activated with 100 units/ml of human rIL-2 for 72 hr. However, the degree of the suppression of colony formation by lymphocytes activated with rIL-2 was different for each cell line. The per cent inhibition of colony formation obtained by HTCA correlated well with the per cent cytolysis obtained by 51Cr-release assay for all cell lines. HTCA provides a very useful tool for the detection of cytotoxicity of lymphocytes against clonogenic tumor cells.

• Authors: J Fujita, N Saijo, Y Sasaki, H Futami, J Ishihara, H Takahashi, A Hoshi, A W Hamburger
• Journal: European journal of cancer & clinical oncology (Eur J Cancer Clin Oncol) Vol. 22 Issue 4 Pg. 445-50 (Apr 1986) ISSN: 0277-5379 [Print] England
• PMID: 3488219 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-2

Topics

• Cell Division
• Cell Line
• Cytotoxicity, Immunologic
• Humans
• Interleukin-2 (immunology)
• Killer Cells, Natural (immunology)
• Lung Neoplasms (immunology, pathology)
• Lymphocyte Activation
• Tumor Stem Cell Assay