Cell cycle progression and SCE rate of Bloom syndrome cells with/without co-cultivation in the presence/absence of normal cells.

Abstract

The present study was undertaken to examine cell cycle progression and SCE rate in three types of B-lymphoid cell line, viz., normal (KS-86), high-SCE Bloom syndrome (BS (BS2-2) and dimorphic BS (BS-SYW). In order to compare the dimorphic condition (BS-SYW) with artificial dimorphism (co-cultivation of BS2-2 with KS-86) these experiments were designed to test whether the BS B-lymphoid cell line cultures would influence the cell cycle progression and SCE rates of a normal B-lymphoid cell line, and vice versa. The present study resolved the controversy reported in the literature, by finding a definite time period under co-cultivation conditions when the SCE in normal cells was increased after 8 days of co-culture, whereas SCE in the BS cells decreased immediately with co-cultivation. In the dimorphic BS cell line (BS-SYW) the SCE frequency of a high-SCE cell population was also observed to be lower than that of a non-dimorphic BS cell line (BS2-2), thus corroborating the experimental observations under co-cultivation conditions. The decrease in BS SCE and increase in normal SCE (after a particular time period) is attributed to numerous causes discussed in relation to the cell cycle progression.

• Authors: R Bamezai, Y Shiraishi
• Journal: Experimental cell research (Exp Cell Res) Vol. 164 Issue 1 Pg. 163-73 (May 1986) ISSN: 0014-4827 [Print] United States
• PMID: 3485532 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• B-Lymphocytes (cytology)
• Bloom Syndrome (genetics, pathology)
• Cell Cycle
• Cell Line
• Humans
• Kinetics
• Mitotic Index
• Sister Chromatid Exchange