Abstract |
A germline T-cell receptor variable region (V beta) gene segment (V beta 14) has been mapped 10 kilobases to the 3' side of the constant region (C beta 2) gene. The V beta 14 gene segment is in an inverted transcriptional polarity relative to the diversity-region (D beta) and joining-region (J beta) gene segments and the C beta genes. Analyses of a T-cell clone (J 6.19), which has productively rearranged the V beta 14 gene segment, indicate that the productive V beta-D beta-J beta rearrangement and its reciprocal flank recombination product are linked and located at either border of a chromosomal inversion. These data demonstrate for the first time a linkage between mammalian V and C genes and verify that a functional T-cell receptor V beta gene can be constructed through a chromosomal inversion.
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Authors | M Malissen, C McCoy, D Blanc, J Trucy, C Devaux, A M Schmitt-Verhulst, F Fitch, L Hood, B Malissen |
Journal | Nature
(Nature)
1986 Jan 2-8
Vol. 319
Issue 6048
Pg. 28-33
ISSN: 0028-0836 [Print] England |
PMID | 3484541
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Receptors, Antigen, T-Cell
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Topics |
- Amino Acid Sequence
- Cell Differentiation
- Chromosome Inversion
- Genes
- Germ Cells
(physiology)
- Humans
- Receptors, Antigen, T-Cell
(genetics)
- Recombination, Genetic
- T-Lymphocytes
(physiology)
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