Direct evidence for chromosomal inversion during T-cell receptor beta-gene rearrangements.

Abstract

A germline T-cell receptor variable region (V beta) gene segment (V beta 14) has been mapped 10 kilobases to the 3' side of the constant region (C beta 2) gene. The V beta 14 gene segment is in an inverted transcriptional polarity relative to the diversity-region (D beta) and joining-region (J beta) gene segments and the C beta genes. Analyses of a T-cell clone (J 6.19), which has productively rearranged the V beta 14 gene segment, indicate that the productive V beta-D beta-J beta rearrangement and its reciprocal flank recombination product are linked and located at either border of a chromosomal inversion. These data demonstrate for the first time a linkage between mammalian V and C genes and verify that a functional T-cell receptor V beta gene can be constructed through a chromosomal inversion.

• Authors: M Malissen, C McCoy, D Blanc, J Trucy, C Devaux, A M Schmitt-Verhulst, F Fitch, L Hood, B Malissen
• Journal: Nature (Nature) 1986 Jan 2-8 Vol. 319 Issue 6048 Pg. 28-33 ISSN: 0028-0836 [Print] England
• PMID: 3484541 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Receptors, Antigen, T-Cell

Topics

• Amino Acid Sequence
• Cell Differentiation
• Chromosome Inversion
• Genes
• Germ Cells (physiology)
• Humans
• Receptors, Antigen, T-Cell (genetics)
• Recombination, Genetic
• T-Lymphocytes (physiology)