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Determination of 9 alpha, 11 beta-prostaglandin F2 by stereospecific antibody in various rat tissues.

Abstract
In view of the recent finding that prostaglandin D2 is stereospecifically converted to 9 alpha, 11 beta-prostaglandin F2, an isomer of prostaglandin F2 alpha, a highly specific and sensitive radioimmunoassay for 9 alpha, 11 beta-prostaglandin F2 was developed and applied to determine the content of this prostaglandin in various rat tissues. Antisera against 9 alpha, 11 beta-prostaglandin F2 were raised in rabbits immunized with the bovine serum albumin conjugate, and [3H]9 alpha, 11 beta-prostaglandin F2 was enzymatically prepared from [3H]prostaglandin D2. The assay detected 9 alpha, 11 beta-prostaglandin F2 over the range of 20 pg to 1 ng, and the antiserum showed less than 0.04% cross-reaction with prostaglandin F2 alpha, prostaglandin F2 beta and 9 beta, 11 beta-prostaglandin F2. To avoid postmortem changes, tissues were frozen in liquid nitrogen immediately after removal. The basal level of 9 alpha, 11 beta-prostaglandin F2 was hardly detectable in various tissues of the rat examined, including spleen, lung, liver and brain; although it was found to be 0.31 +/- 0.06 ng/g wet weight in the small intestine. During convulsion induced by pentylenetetrazole, enormous amounts of prostaglandin D2 (ca. 180 ng/g wet weight) and prostaglandin F2 alpha (ca. 70 ng/g) were produced in the brain; however, 9 alpha, 11 beta-prostaglandin F2 was detected neither there nor in the blood. This result demonstrates that the conversion to 9 alpha, 11 beta-prostaglandin F2 is a minor pathway, if one at all, of prostaglandin D2 metabolism in the rat brain.
AuthorsH Hayashi, S Ito, T Tanaka, M Negishi, H Kawabe, H Yokohama, K Watanabe, O Hayaishi
JournalProstaglandins (Prostaglandins) Vol. 33 Issue 4 Pg. 517-30 (Apr 1987) ISSN: 0090-6980 [Print] United States
PMID3474704 (Publication Type: Journal Article)
Chemical References
  • Immune Sera
  • Prostaglandins D
  • Prostaglandins F
  • Dinoprost
  • Prostaglandin D2
  • Pentylenetetrazole
Topics
  • Animals
  • Chromatography, High Pressure Liquid
  • Dinoprost
  • Immune Sera
  • Male
  • Pentylenetetrazole (pharmacology)
  • Prostaglandin D2
  • Prostaglandins D (analysis)
  • Prostaglandins F (analysis, chemical synthesis, metabolism)
  • Radioimmunoassay (methods)
  • Rats
  • Rats, Inbred Strains
  • Seizures (metabolism)
  • Tissue Distribution

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