Specific serodiagnosis with adult Onchocerca volvulus antigen in an enzyme-linked immunosorbent assay.

Abstract

The cross-reactivity of the blood from onchocerciasis, loiasis, and dipetalonemiasis was tested by a micro-ELISA technique, utilizing adult Onchocerca volvulus antigen and blood samples taken on filter paper. The average ELISA values (OD at 500 nm) were as follows: 0.58 in persons with O. volvulus microfilariae (n = 81), 0.49 in microfilariae-negatives from the same endemic area (n = 39), 0.15 in dipetalonemiasis (n = 27), and 0.25 in loiasis (n = 12), while those of 65 Dipetalonema perstans-negative people were markedly low (average 0.14) and that of 22 Loa loa-negatives, 0.22, respectively. This ELISA could successfully differentiate onchocerciasis from dipetalonemiasis and loiasis.

• Authors: I Tada, M Korenaga, K Shiwaku, E O Ogunba, G O Ufomadu, B E Nwoke
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 36 Issue 2 Pg. 383-6 (Mar 1987) ISSN: 0002-9637 [Print] United States
• PMID: 3469920 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Helminth

Topics

• Adolescent
• Adult
• Aged
• Antigens, Helminth (analysis)
• Dipetalonema Infections (diagnosis)
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Loiasis (diagnosis)
• Male
• Middle Aged
• Onchocerca (immunology)
• Onchocerciasis (diagnosis, immunology)
• Serologic Tests