Marked granulocytosis in C57BL/6 mice bearing a transplanted BMT-11 fibrosarcoma.

Abstract

When a 3-methylcholanthrene (CAS: 56-49-5)-induced fibrosarcoma, BMT-11, and its eight clones were transplanted sc into syngeneic C57BL/6 mice, leukemoid reaction characterized by a progressive increase in peripheral white blood cells (WBCs) and by splenomegaly was observed as the tumors grew. The WBC count reached about forty-fold of the normal level, and more than 90% of WBCs were found to be polymorphonuclear leukocytes. The increase in WBCs was correlated with tumor size, and its count decreased to normal level within 7 days after surgical excision of subcutaneous tumors. Moreover, a high level of colony-stimulating activity was detected in the supernatant of BMT-11 culture. These results suggest that the colony-stimulating factor produced by BMT-11 cells caused granulocytosis in mice. This is the first report of the marked degree of granulocytosis induced by a transplanted tumor in C57BL/6 strain mice.

• Authors: M Ishikawa, M Hosokawa, N Oh-hara, Y Niho, H Kobayashi
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 78 Issue 3 Pg. 567-71 (Mar 1987) ISSN: 0027-8874 [Print] United States
• PMID: 3469467 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Methylcholanthrene

Topics

• Animals
• Bone Marrow (pathology)
• Colony-Forming Units Assay
• Female
• Fibrosarcoma (chemically induced, pathology)
• Granulocytes (pathology)
• Leukocyte Count
• Macrophages (pathology)
• Methylcholanthrene
• Mice
• Mice, Inbred C57BL
• Neoplasm Transplantation
• Splenectomy