Retinoic acid-induced monocytic differentiation of HL60/MRI, a cell line derived from a transplantable HL60 tumor.

Abstract

The human promyelocytic leukemia cell line HL60 differentiates to either granulocytes or monocytes/macrophages when induced with various chemicals and lymphokines. Retinoic acid (RA) induces HL60 to differentiate to granulocyte-like cells. However, HL60/MRI cells, derived from a transplantable HL60 tumor established in athymic nude mice, differentiate to monocytoid cells when cultured with RA in vitro. HL60/MRI induced with RA are monocytes based on morphology and the expression of markers and functions specific for monocytes such as: the OKM5 monocyte-specific antigen, nonspecific esterase activity, and adhesiveness. HL60/MRI is much more sensitive to RA than is HL60. Thus, the RA concentrations that induce 50% differentiation are 0.41 nM for HL60/MRI and 37 nM for HL60, and maximum differentiation occurs at 2 days for HL60/MRI and at 4 days for HL60. While RA induces HL60/MRI to monocytoid cells, other inducers of granulocytic differentiation of HL60, such as dimethyl sulfoxide and hexamethylene bisacetamide, induce HL60/MRI to granulocytes. Furthermore, 12-O-tetradecanoylphorbol-13-acetate induces both HL60 and HL60/MRI to macrophage-like cells. The isozyme phenotypes of HL60/MRI and HL60 are identical. Cytogenetic analysis of HL60/MRI indicates that many of its normal chromosomes are triploid and that it has five abnormal chromosome markers, M1-M5, three of which, M1-M3, are seen also in HL60. This unique cell line, HL60/MRI, may be useful for studying the event(s) triggering differentiation of myelomonocytic cells and the mechanism of action of RA.

• Authors: M Imaizumi, J Uozumi, T R Breitman
• Journal: Cancer research (Cancer Res) Vol. 47 Issue 5 Pg. 1434-40 (Mar 01 1987) ISSN: 0008-5472 [Print] United States
• PMID: 3469018 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Acetamides
• Antigens, Surface
• Isoenzymes
• Tretinoin
• hexamethylene bisacetamide
• Tetradecanoylphorbol Acetate
• Dimethyl Sulfoxide

Topics

• Acetamides (pharmacology)
• Animals
• Antigens, Surface (analysis)
• Cell Differentiation (drug effects)
• Cell Line
• Chromosome Aberrations
• Dimethyl Sulfoxide (pharmacology)
• Humans
• Isoenzymes (analysis)
• Leukemia, Myeloid, Acute (genetics, pathology)
• Mice
• Mice, Inbred BALB C
• Monocytes (immunology, pathology)
• Neoplasm Transplantation
• Tetradecanoylphorbol Acetate (pharmacology)
• Tretinoin (pharmacology)