Separation and properties of rabbit buccal mucosal wound hyaluronidase.

Abstract

This study establishes the existence of a mammalian buccal mucosal wound hyaluronidase (hyaluronate 4-glycohydrolase; EC 3.2.1.35) having properties distinct from those of the endogenous lysosomal hyaluronidase of normal (uninjured) buccal mucosa. A time-dependent change in hyaluronidase activity was measured, with the highest specific activity occurring on post-wound day 4 (7.7 +/- 1.3 units/mg protein), followed by consecutive decreases until activity was no longer discernible by day 21. Mucosal wound hyaluronidase closely resembled a previously described integumentary wound endoglycosidase in terms of a high pH optimum (5.0-6.0), distinct (but non-exclusive) substrate preference for hyaluronic acid, and ability to generate saturated depolymerization products by an endoglycosidic hydrolysis.

• Authors: C N Bertolami, R H Day, D G Ellis
• Journal: Journal of dental research (J Dent Res) Vol. 65 Issue 6 Pg. 939-44 (Jun 1986) ISSN: 0022-0345 [Print] United States
• PMID: 3458746 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Hyaluronoglucosaminidase

Topics

• Animals
• Cheek
• Chromatography, Ion Exchange
• Chromatography, Paper
• Colorimetry
• Granulation Tissue (enzymology)
• Hyaluronoglucosaminidase (isolation & purification)
• Mouth Mucosa (enzymology, injuries)
• Rabbits
• Wound Healing