Abstract |
Tritiated [(16 alpha-ethyl-21-hydroxy-19-nor-pregn-4-ene-3,20-dione)-6,7-3H] (ORG-2058) and 17,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione ( R5020) were compared as ligands in the assay of progesterone receptor in human and rat breast tumors. We found that ORG-2058 is a better ligand because of its low nonspecific binding. Most of the nonspecific binding of the other ligand R5020, is to proteins which bind corticosteroids. In cancerous tissue ORG-2058 binds to progesterone receptor linearly in a range of protein concentrations which are normally used in the receptor assay. On the other hand, R5020 exhibits binding linearity over a narrower protein concentration in many tumor biopsies, which may cause severe limitation in the assay procedure or frequent underestimation of receptor content.
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Authors | Y Sharoni, B Feldman, N Karny, J Levy |
Journal | Steroids
(Steroids)
1986 Nov-Dec
Vol. 48
Issue 5-6
Pg. 419-26
ISSN: 0039-128X [Print] United States |
PMID | 3445291
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Pregnenediones
- Receptors, Progesterone
- 16 alpha-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione
- Promegestone
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Topics |
- Animals
- Breast Neoplasms
(metabolism)
- Cytosol
(metabolism)
- Female
- Humans
- Male
- Mammary Neoplasms, Experimental
(metabolism)
- Pregnenediones
- Promegestone
- Rats
- Receptors, Progesterone
(analysis)
- Uterus
(metabolism)
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