Enhanced expression of Harvey-ras oncogene in FANFT-induced transitional cell carcinoma.

Abstract

The level of mRNA expression and DNA structure of c-H-ras were examined in a transitional cell carcinoma (TCC) induced in C3H/He mice and Fisher rats by N-(4,5-nitro-2-furylthiazolyl)formamide (FANFT) and carried as serially transplanted tumors. Neither amplification nor gross rearrangements were detected by Southern analysis in the FANFT tumor. Increased expression of c-H-ras was detected by Northern and in situ hybridization in both the FANFT-induced mouse and rat TCC, as compared to normal urothelium. No expression of six other cellular oncogenes was detected in the tumor. It appears that the increase in H-ras expression in the FANFT tumor is specific to this oncogene and that increased expression of the H-ras gene may play a role in bladder carcinogenesis.

• Authors: I S Sawczuk, W Walsh, W King, C A Olsson, C Nguyen-Huu
• Journal: Urologia internationalis (Urol Int) Vol. 42 Issue 5 Pg. 321-5 ( 1987) ISSN: 0042-1138 [Print] Switzerland
• PMID: 3433577 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• RNA, Messenger
• FANFT

Topics

• Animals
• Carcinoma, Transitional Cell (chemically induced, genetics)
• FANFT
• Gene Expression Regulation
• Mice
• Mice, Inbred Strains
• Neoplasm Transplantation
• Oncogenes
• RNA, Messenger (genetics)
• Rats
• Rats, Inbred F344
• Urinary Bladder Neoplasms (chemically induced, genetics)