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Induction of synthesis of tumor necrosis factor in human and murine cell lines by exogenous recombinant human tumor necrosis factor.

Abstract
Treatment of sensitive human myosarcoma cells (KYM-S) with exogenous tumor necrosis factor (r-TNF) resulted in the production of TNF by the cells. The newly synthesized cellular TNF was identified immunologically on Western blots and as a single 1.8-kilobase band on Northern blots. TNF synthesis began within 2 h of administration of the exogenous TNF in a dose-dependent manner. r-TNF also induced TNF synthesis in mouse tumorigenic fibroblasts (L-M). Resistant sublines of these cells as well as TNF nonsensitive human diploid fibroblasts possessed TNF mRNA without pretreatment, indicating an inverse correlation between levels of TNF expressed and sensitivity to the cytotoxic effects of exogenous TNF. It is conceivable that the newly synthesized cellular TNF functions in some protective manner to block cytolytic effects of exogenous TNF.
AuthorsY Niitsu, N Watanabe, H Neda, N Yamauchi, M Maeda, H Sone, H Kuriyama
JournalCancer research (Cancer Res) Vol. 48 Issue 19 Pg. 5407-10 (Oct 01 1988) ISSN: 0008-5472 [Print] United States
PMID3416298 (Publication Type: Journal Article)
Chemical References
  • RNA, Messenger
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
Topics
  • Animals
  • Cell Line
  • HeLa Cells (metabolism)
  • Humans
  • Kinetics
  • Mice
  • Myosarcoma (pathology)
  • RNA, Messenger (metabolism)
  • Recombinant Proteins (pharmacology)
  • Tumor Necrosis Factor-alpha (biosynthesis, genetics)

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