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The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E.

Abstract
Lipid peroxidation of rat liver microsomal fractions was monitored by its low-level chemiluminescence in preparations from controls and vitamin-E-deficient animals. Measurements were made (a) of the duration of the lag phase tau0 after initiation with NADPH/iron-ADP and (b) of the slope of the chemiluminescence increase. In microsomes with normal vitamin E (alpha-tocopherol) level the lag phase tau0 was substantially increased by ascorbate; in contrast, even an enhanced peroxidation was observed with ascorbate in vitamin-E-deficient microsomes. Therefore, the ascorbate-mediated protection of microsomal membranes against lipid peroxidation is dependent on vitamin E in the membrane. In vitamin E deficiency the pro-oxidant effect of ascorbate was abolished when glutathione (GSH) was present. Likewise, GSH does not prolong the lag phase tau0 in vitamin E deficiency. However, GSH (but not cysteine) exerts an antioxidant effect both in controls and in vitamin E deficiency by decreasing the slope of the chemiluminescence increase during lipid peroxidation. The involvement of GSH in an enzyme-dependent mechanism is suggested.
AuthorsH Wefers, H Sies
JournalEuropean journal of biochemistry (Eur J Biochem) Vol. 174 Issue 2 Pg. 353-7 (Jun 01 1988) ISSN: 0014-2956 [Print] England
PMID3383850 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Lipid Peroxides
  • Sulfhydryl Compounds
  • Vitamin E
  • Glutathione
  • Cysteine
  • Ascorbic Acid
Topics
  • Animals
  • Ascorbic Acid (pharmacology)
  • Cysteine (pharmacology)
  • Glutathione (pharmacology)
  • Lipid Peroxides (biosynthesis)
  • Luminescent Measurements
  • Microsomes, Liver (metabolism)
  • Rats
  • Sulfhydryl Compounds (pharmacology)
  • Vitamin E (pharmacology)
  • Vitamin E Deficiency (metabolism)

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