Changes in intracellular ion activities induced by adrenaline in human and rat skeletal muscle.

Abstract

To study the stimulating effect of adrenaline (ADR) on active Na+/K+ transport we used double-barrelled ion-sensitive micro-electrodes to measure the activities of extracellular K+ (aKe) and intracellular Na+ (aNai) in isolated preparations of rat soleus muscle, normal human intercostal muscle and in one case of hyperkalemic periodic paralysis (h.p.p.). In these preparations, bath-application of ADR (10(-6) M) resulted in a membrane hyperpolarization and transient decreases aKe and aNai which could be blocked by ouabain (3 x 10(-4) M). In the h.p.p. muscle a continuous rise of aNai induced by elevation of aKe to 5.2 mM could be stopped by ADR. In addition, the intracellular K+ activity (aKi), the free intracellular Ca2+ concentration (pCai) and intracellular pH (pHi) were monitored in rat soleus muscle. During ADR aKi increased, pHi remained constant and intracellular Ca2+ apparently decreased. In conclusion, our data show that ADR primarily stimulates the Na+/K+ pump in mammalian skeletal muscle. This stimulating action is not impaired in the h.p.p. muscle.

• Authors: K Ballanyi, P Grafe
• Journal: Pflugers Archiv : European journal of physiology (Pflugers Arch) Vol. 411 Issue 3 Pg. 283-8 (Mar 1988) ISSN: 0031-6768 [Print] Germany
• PMID: 3380643 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Sodium
• Potassium
• Calcium
• Epinephrine

Topics

• Animals
• Biological Transport, Active
• Calcium (metabolism)
• Epinephrine (pharmacology)
• Humans
• Hydrogen-Ion Concentration
• Hyperkalemia (metabolism)
• Male
• Muscles (drug effects, metabolism)
• Paralysis (metabolism)
• Potassium (metabolism)
• Rats
• Sodium (metabolism)