Effect of endotoxin on the rat colon glutathione level.

Abstract

The effect of endotoxin on the colon glutathione level was studied in male rats. Endotoxin (Escherichia coli) from 25 ug to 100 ug/100g body weight was administered intravenously. The Glutathione level was measured 16 hours after endotoxin was given. Results showed that endotoxin significantly enhanced the colon glutathione concentration as measured by 5,5'-dithiobis (2-nitrobenzoic acid). The increase which ranged from 11% to 50% was dose dependent. At an endotoxin dose of 1000 ug/100g body weight, colon glutathione level was found to be enhanced from 2 hours up to 48 hours. In contrast, the duodenum and jejunum glutathione levels were found to be significantly reduced. The increase in the colon glutathione level may have a protective effect against oxidative damage to the colon.

• Authors: M F Chen, L T Chen, H W Boyce Jr
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 151 Issue 2 Pg. 844-50 (Mar 15 1988) ISSN: 0006-291X [Print] United States
• PMID: 3348815 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Endotoxins
• Glutathione

Topics

• Animals
• Colon (drug effects, metabolism)
• Duodenum (drug effects, metabolism)
• Endotoxins (pharmacology)
• Fasting
• Glutathione (metabolism)
• Intestinal Mucosa (drug effects, metabolism)
• Jejunum (drug effects, metabolism)
• Kinetics
• Male
• Muscles (drug effects, metabolism)
• Rats
• Rats, Inbred Strains
• Reference Values