HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

The influence of urinary macromolecules on calcium oxalate monohydrate crystal growth.

Abstract
The Constant Composition (CC) kinetics method has been used for studying the mineralization of calcium oxalate monohydrate (COM) at sustained supersaturations in the presence of pre-bladder urine and macromolecules isolated from normal urine and kidney and bladder stones. The method is especially sensitive for investigating the inhibitory activities of these urinary macromolecular components (UMMC) and matrix macromolecular components (MMMC) with a coefficient of variation in growth rate of approximately 2%. Significant COM mineral inhibition was observed in a wide molecular weight region of urine components. Urine removed directly from the kidney showed appreciable inhibitory activity towards COM crystallization. Normal urinary proteins and the dissolved precipitate resulting from urine centrifugation were fractionated by gel filtration. The resulting solutions were mostly COM mineralization inhibitors. Electrodialysis was utilized to isolate the MMMC (greater than 7000 d) of renal and bladder calculi. While these solutions inhibited COM crystallization, they were also found to be calcium binders as measured by the calcium electrode.
AuthorsA C Lanzalaco, R P Singh, S A Smesko, G H Nancollas, G Sufrin, M Binette, J P Binette
JournalThe Journal of urology (J Urol) Vol. 139 Issue 1 Pg. 190-5 (Jan 1988) ISSN: 0022-5347 [Print] United States
PMID3336094 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Glycoproteins
  • Macromolecular Substances
  • nephrocalcin
  • Calcium Oxalate
Topics
  • Calcium Oxalate (antagonists & inhibitors, urine)
  • Chemical Precipitation
  • Chromatography, Gel
  • Crystallization
  • Glycoproteins (urine)
  • Humans
  • Kidney Calculi (analysis, metabolism)
  • Macromolecular Substances (analysis)
  • Urinary Bladder Calculi (analysis, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: