Protein synthesis in polyamine-deficient bacteria during amino-acid starvation.

Abstract

Amino-acid starvation in polyamine-auxotrophic bacteria grown in the presence of putrescine provokes a marked inhibition of protein synthesis. This inhibition is almost completely relieved in polyamine-depleted cells. The differential behaviour of bacterial protein synthesis depending on the endogenous levels of polyamines is not due to a change in the uptake of amino acids used to measure protein synthesis, nor to the decreased growth rate of polyamine-depleted cells. During leucine starvation, cells grown with putrescine synthesized a somewhat lower amount of high-molecular-weight proteins than polyamine-depleted bacteria. In addition, cells with normal endogenous levels of polyamines accumulated significant amounts of 62 and 41 kDa polypeptides as well as several low-molecular-weight peptides.

• Authors: H G Nastri, I D Algranati
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 949 Issue 1 Pg. 65-70 (Jan 25 1988) ISSN: 0006-3002 [Print] Netherlands
• PMID: 3275465 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids
• Bacterial Proteins
• Polyamines
• Putrescine

Topics

• Amino Acids (metabolism)
• Bacterial Proteins (biosynthesis)
• Biological Transport (drug effects)
• Escherichia coli (drug effects, genetics, metabolism)
• Kinetics
• Molecular Weight
• Polyamines (metabolism)
• Putrescine (pharmacology)