Production of tumor necrosis factor by human monocytes in response to toxic-shock-syndrome toxin-1.

Abstract

We studied the effect of toxic-shock-syndrome toxin-1 (TSST-1) on production of tumor necrosis factor (TNF) by human monocytes. Adherent mononuclear cells were stimulated with TSST-1 and their supernatants assayed for TNF by using L929 cells in a cytotoxicity assay. TSST-1 stimulated production of TNF over a wide range of concentrations. The cytotoxicity of monocyte supernatants was neutralized by antibody to TNF but not by antibody to interleukin-1 or by normal rabbit serum. TSST-1 and lipopolysaccharide (LPS) had a synergistic effect on monokine production. Monocytes "primed" with TSST-1 produced more interleukin-1 and TNF in response to LPS than did unprimed cells. Treating monocytes with LPS before TSST-1 and co-incubating the two agents with cells for 24 h also enhanced monokine production under some circumstances. These studies suggest a role for TNF in the pathogenesis of toxic shock syndrome, as a consequence of induction by TSST-1 alone or the synergistic effects of several bacterial products.

• Authors: J Parsonnet, Z A Gillis
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 158 Issue 5 Pg. 1026-33 (Nov 1988) ISSN: 0022-1899 [Print] United States
• PMID: 3263447 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Toxins
• Enterotoxins
• Interleukin-1
• Lipopolysaccharides
• Superantigens
• Tumor Necrosis Factor-alpha
• enterotoxin F, Staphylococcal

Topics

• Bacterial Toxins
• Cell Adhesion
• Cell Line
• Cells, Cultured
• Cytotoxicity Tests, Immunologic
• Drug Synergism
• Enterotoxins (pharmacology)
• Humans
• Interleukin-1 (biosynthesis)
• Lipopolysaccharides (pharmacology)
• Monocytes (immunology, metabolism)
• Staphylococcus aureus
• Superantigens
• Tumor Necrosis Factor-alpha (biosynthesis)